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Sand substrate 200 ml
With a simple 'test-tube-in-a-box' setup it's often not a bad idea to add a thin layer of clear sand, adding benefits like ease of cleaning, stability and helping out the ants in some ways..
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Cocos substrate 2 liter
The most popular substrate material for ants because of it's water-retention capabilities. Sold as solid 'peat' blocks, just soak it in water to loosen it up and make itl expand enormously.
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Hempfiber substrate 200 gram
This coarse substrate is favored for nest building by ants like Polyrhachis dives and Formica rufa. It's also a clean option for a natural outworld substrate layer.